Sampletype i-sep® DL-MB
Sampletype i-sep® DL-MB Kit
Features
- Efficient and reproducible separation of DNA fractions during differential lysis
- Fast and easy to perform DNA preparation in the same device without extra pipetting steps
- Minimized risk of cross-contaminations and mix-ups (no manual substrate transfer)
- Ethylene oxide sterilized to guarantee a RNA, DNA, RNase and DNase free product
- Increased effectiveness of the DNA extraction due to the lysis buffer Sampletype i-sep® MB
The success of an optimal separation of DNA derived from victim and offender hinges on an efficient differential lysis. Vaginal smears derived from sexual offences are particularly challenging due to the large number of female epithelial cells compared to the small number of sperm cells. The method relies on the differential lysis of epithelial cells and sperm cells by an optimized proprietary buffer. The combination of the buffer Sampletype i-sep® MB with the i-sep® DL Spin Column provides the perfect solution to this problem. The proprietary filter material allows the lysis and separation to be carried out in the same device and without extra pipetting steps. For a subsequent DNA purification, manual or automated methods can be used.
Product Specifications
- Maximum filling volume: 200 - 500 µL
- Maximum centrifugation force: 5,214 g / r = 95 mm (7,000 rpm)
- Temperature stability: ≤ 60 ˚C
- Stain material: e. g. swabs, cloth, tissue, FFPE embedded material
Product References
- White, T. J. et al. Developmental validation of an efficient differential separation method incorporating the i-sep® DL spin column with high sperm DNA recovery for the processing of sexual assault samples. Journal of Forensic Science 67, 1679-1689. https://doi.org/10.1111/1556-4029.15043 (2022)
- Vieira da Silva, C. et al. Avaliação da eficácia da lise diferencial em amostras biológicas de processos sexuais. http://hdl.handle.net/10400.26/37346 (2019)
- Bogas, V. et al. Validation of sampletype I-sep DL for differential extraction and purification with prepfiler express in the automate express DNA extraction system. Forensic Science International: Genetics Supplement Series 6, e353-e354. https://doi.org/10.1016/j.fsigss.2017.09.113 (2017)
- Schwedtner, G. et al. The separation of male and female: A comparison of seven protocols (P). Forensic Science International: Genetics Supplement Series 6, e9-e11. https://doi.org/10.1016/j.fsigss.2017.09.021 (2017)
Resources
Ordering Information
Sampletype i-sep® DL-MB
Size: 10 reactions
Cat. No.: 70-13701-0010
Status: RUO*
Sampletype i-sep® DL-MB
Size: 100 reactions
Cat. No.: 70-13701-0100
Status: RUO*
Sampletype i-sep® DL-MB
Size: 250 reactions
Cat. No.: 70-13701-0250
Status: RUO*
Sampletype i-sep® DL-MB (without Lysepuffer)
Size: 250 reactions
Cat. No.: 70-13705-0250
Status: RUO*
*RUO - Research Use Only, not for diagnostic purposes.
Product Request
MODAPLEX
Bench-top system for molecular multi-marker testing
