Mentype® DIPscreen

Tailored chimerism monitoring

Features

High discrimination power
Low DNA input for high sensitivity ≥ 2 %
Allows high throughput genotyping and monitoring

Mentype^® DIPscreen is the optimal tool for a cost-effective start into sensitive chimerism analysis. The kit is used for initial genotyping of recipient and donor samples and can also be used for the routine chimerism monitoring with a sensitivity of up to 2 %. A subsequent highly sensitive chimerism quantification can be performed either with the qPCR assays Mentype^® DIPquant or the broad applicable digital PCR kit Mentype^® DigitalQuant (RUO). These combined approaches provide flexible and reliable chimerism monitoring adaptable to your needs.

Biomarkers

‍

FAM-Panel

DIP Locus: AM X, AM Y, HLD106, HLD70, HLD84, HLD103, HLD104, HLD116, HLD112, HLD307, HLD310, HLD110, HLD133, HLD79, HLD105,HLD140, HLD163

‍Chromosomal localization: Xp22.1-22.3, Yp11.2, 16q13, 6q16.1, 8q24.12, 12q23.1, 13q32.1, 18p11.22, 17p12, Xp11.23, 2p22.3, 16q22.1, 3p22.1, 7q31.2, 14q24.3, 3q23, 12q24.31

‍

BTG Panel

DIP Locus: HLD91, HLD23, HLD88, HLD101, HLD67, HLD301, HLD53, HLD97, HLD152, HLD128, HLD134, HLD305

‍Chromosomal localization:11q14.1, 18p11.32, 9q22.33, 15q26.1, 5q33.3, 17q21.32, 3q22.1, 13q13.1, 16p13.2, 1q31.3, 5q11.2, 20q11.22

‍

BTY Panel

DIP Locus: HLD48, HLD114, HLD304, HLD131, HLD38, HLD82

‍Chromosomal localization: 2q11.2, 17p13.2, 9q34.3, 7q36.2, 1q32.2, 7q21.3

Product Specifications

Panel
33 DIP Loci + Amelogenin

Reactions
1 Multiplex PCR reaction per sample

PCR controls
2 included (PC, NTC)

Sample input
1-2 ng gDNA from peripheral blood

Sensitivity
≥ 2 %

Turnaround time
~ 4 h after nucleic acid preparation

Detection
Qualitative genotyping, Semiquantitative chimerism detection

To be used with
Standard thermal cycler + Thermo Fisher Genetic Analyzer

Data analysis
ChimerisMonitor (RUO), GeneMapperTM ID/ ID-X + specific templates

Scientific Background

Analysis of molecular chimerism resulting from allogeneic stem cell transplantation has become a well-established method to control the course of transplant engraftment and to assess the risk of threatening relapse. Molecular chimerism analysis can be performed on diverse DNA-sequence motifs, of which biallelic short insertion/deletion polymorphisms (DIPs, INDELs) offer substantial benefits. Polymerase-mediated amplification of DIP-markers does not result in formation of stutter peaks that can hamper clear analysis. Moreover, these polymorphisms are best suited for allele-specific quantitative approaches. Mentype^® DIPscreen is a DIP-based chimerism analysis and therefore accounts for an unambiguous donor/recipient differentiation and highly clear chimerism monitoring.

Product References

Ferreras, C. et al. Results of phase 2 randomized multi-center study to evaluate the safety and efficacy of infusion of memory T cells as adoptive therapy in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia and/or lymphopenia (RELEASE NCT04578210). Cytotherapy 26, 25-35, https://doi.org/10.1016/j.jcyt.2023.10.002 (2024)
Al-Akioui Sanz, K. et al. Familial CD45RA– T cells to treat severe refractory infections in immunocompromised patients. Front. Med. 10. https://doi.org/10.3389/fmed.2023.1083215 (2023)
Huyveneers, L. E. P. et al. Autopsy Study Defines Composition and Dynamics of the HIV-1 Reservoir after Allogeneic Hematopoietic Stem Cell Transplantation with CCR5D32/D32 Donor Cells. Viruses 14. https://doi.org/10.3390/v14092069 (2022)
Pérez-Martínes, A. et al. Phase I dose-escalation single centre clinical trial to evaluate the safety of infusion of memory T cells as adoptive therapy in COVID-19 (RELEASE). EClinicalMedicine 39. https://doi.org/10.1016/j.eclinm.2021.101086 (2021)
Fortschegger, M. et al. Detection and Monitoring of Lineage-Specific Chimerism by Digital Droplet PCR-Based Testing of Deletion/Insertion Polymorphisms. Biol Blood Marrow Transplant 26, 1218-1224, https://doi.org/10.1016/j.bbmt.2020.02.016 (2020)
Gómez-García, L. M. et al. A phase II clinical trial of infusing haploidentical K562-mb-IL15-41BBL activated and expanded Natural Killer cells as consolidation therapy for pediatric acute myeloblastic leukemia. ESS Open Archive. DOI: 10.22541/au.160192968.85891275/v1 (2020)
Navarro-Bailón, A et al. Short Tandem Repeats (STRs) as Biomarkers for the Quantitative Follow-Up of Chimerism after Stem Cell Transplantation: Methodological Considerations and Clinical Application. Genes 11, 993. https://doi.org/10.3390/genes11090993 (2020)
Cechova, H. et al. Suitable Molecular Genetic Methods for the Monitoring of Cell Chimerism. Rare Diseases. IntechOpen. http://dx.doi.org/10.5772/intechopen.88436 (2019)
Salgado, M. et al. Mechanisms That Contribute to a Profound Reduction of the HIV-1 Reservoir After Allogeneic Stem Cell Transplant. Annals of Internal Medicine 169, 674-683. https://doi.org/10.7326/M18-0759 (2018)
Sellmann, L. et al. Diagnostic value of highly-sensitive chimerism analysis after allogeneic stem cell transplantation. Bone Marrow Transplant 53, 1457-1465. https://doi.org/10.1038/s41409-018-0176-7 (2018)
Navarro-Bailón, A. et al. A Novel Quantitative PCR Approach Targeting Insertion/Deletion Polymorphisms (Indel-PCR) for Chimerism Quantification: Finally High Sensitivity and Quantification Capacity Together. Blood 126, 4227. https://doi.org/10.1182/blood.V126.23.4277.4277 (2015)
Willasch, A. M. et al. Monitoring of Hematopoietic Chimerism after Transplantation for Pediatric Myelodysplastic Syndrome: Real-Time or Conventional Short Tandem Repeat PCR in Peripheral Blood or Bone Marrow? Biol Blood Marrow Transplant 20, 1918-1925. https://doi.org/10.1016/j.bbmt.2014.07.030 (2014)