Mentype® Chimera® PCR Amplification Kit

The gold standard for STR analysis

Features

Broad applicability - No limitation in mixed chimerism settings
10 % better chance to find highly informative markers (high polymorphic marker)
6 STR markers validated by European Consortium of Chimerism Analysis
Low sample input for highest sensitivity 1-3 %

Mentype^® Chimera^® PCR Amplification Kit was developed as a multiplex application for chimerism monitoring after hematopoietic cell transplantation. Mentype^® Chimera^® offers a reliable semi-quantitative solution for chimerism analysis. With Mentype^® Chimera^®, the genotyping is performed with as little as 200 pg gDNA, and recipient fractions of 1 – 3 % are still detectable during monitoring. Therefore, the assay is the all-rounder for chimerism monitoring.

Biomarkers

Amelogenin X, Amelogenin Y, D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D21S2055, SE33 (ACTBP2)

Product Specifications

Panel
12 STRs + Amelogenin

Reactions
1 multiplex PCR reaction per sample

PCR controls
2 included (PC, NTC)

Sample input
0.2 - 1 ng gDNA from peripheral blood

Sensitivity
1 – 3 %

Turnaround time
~ 4.5 h after nucleic acid preparation

Detection
Qualitative genotyping, Semiquantitative chimerism detection

To be used with
Standard thermal cycler + Thermo Fisher Genetic Analyzer

Data analysis
ChimerisMonitor (RUO), GeneMapper^TM ID/ ID-X + specific templates

Scientific Background

Allogeneic hematopoietic stem cell transplantation (HSCT) is a treatment option to cure patients with non-malignant and malignant hematological diseases, such as leukemia. Chimerism analysis is used to determine the mixture of donor and recipient hematopoietic cells in allo-HSCT recipients to detect early signs of graft rejection. Human peripheral venous blood is used for genotyping and monitoring. Depending on the success of transplantation, different forms of hematopoietic chimerism (complete, mixed or loss) can develop. Different approaches are used for chimerism analysis, including fluorescence in situ hybridization (FISH), restriction fragment length polymorphism (RFLP), blood count analysis and PCR-based methods. Currently, PCR-based amplification of short tandem repeat (STR) polymorphisms is the golden standard for chimerism analysis. To detect early signs of graft rejection, chimerism analysis should be done at regular intervals and shortly after the allogeneic HSCT.

Product References

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