Sampletype i-sep® MB
Sampletype i-sep® MB Buffer
Features
- Highly effective and universal lysis buffer (90 % lysis efficiency)
- Easy to integrate into the existing workflow
- Improved overall efficiency of DNA extraction and resulting DNA yield
- Lysate can easily be processed further with the magnetic beads or with filter columns
Sampletype i-sep® MB is a highly effective and universal lysis buffer for different applications. This buffer can easily be integrated into the existing lab routine. In contrast to other commercial lysis buffers, the Sampletype i-sep® MB is suited for the differential lysis because it selectively prevents an early lysis of sperm cells. With the buffer Sampletype i-sep® MB, even difficult samples like gynecological swabs can easily be processed in combination with the Sampletype i-sep® DL spin columns. For the purification of the lysate, magnetic beads as well as filter columns can be used.
Product Specifications
- Efficient lysis at 56 °C
- Temperature stability: ≤ 95 °C
- Almost complete lysis of epithelial cells within 30 min at 56 °C
- Addition of DTT enables the efficient lysis of sperm cells
Product References
- White, T. J. et al. Developmental validation of an efficient differential separation method incorporating the i-sep® DL spin column with high sperm DNA recovery for the processing of sexual assault samples. Journal of Forensic Science 67, 1679-1689. https://doi.org/10.1111/1556-4029.15043 (2022)
- Bogas, V. et al. Validation of sampletype I-sep DL for differential extraction and purification with prepfiler express in the automate express DNA extraction system. Forensic Science International: Genetics Supplement Series 6, e353-e354. https://doi.org/10.1016/j.fsigss.2017.09.113 (2017)
Resources
Ordering Information
Sampletype i-sep® MB
Size: 20 mL
Cat. No.: 70-13703-0020
Status: RUO*
Sampletype i-sep® MB
Size: 80 mL
Cat. No.: 70-13703-0080
Status: RUO*
Sampletype i-sep® MB
Size: 125 mL
Cat. No.: 70-13703-0125
Status: RUO*
*RUO - Research Use Only, not for diagnostic purposes.
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