Mentype® DigitalScreen
Digital genotyping as prerequisite for clinical routine & research
Features
- Straightforward genotyping
- Streamlined workflow of genotyping & monitoring with one platform
- Performed on standard dPCR systems
- Mid-to-high throughput automation possible depending on dPCR system
The Mentype® DigitalScreen kit serves as a research tool to initial qualitative analysis of the genotype of two human DNA samples. This data enables the measurement of mixed DNA samples, such as in chimerism assessment, utilizing Mentype® DigitalQuant. This integration streamlines the entire genotyping and monitoring process on a single platform.
Biomarkers
Insertion-deletion polymorphism (DIPs or INDELs), SRY, Reference gene
Product Specifications
- Panel
- 30 allele specific duplex assays
- Reactions
- 1 plate for 2 DNA samples
- Internal controls
- Reference gene
- PCR controls
- 2 (PC extra available, NTC)
- Sample input
- 300 ng from peripheral blood
- Turnaround time
- ~ 4.5 h after nucleic acid preparation
- Detection
- Qualitative
- To be used with
- Bio-Rad QX100/200
Scientific Background
The Mentype® Digital approach deploys the highly sensitive digital PCR technology that allows absolute quantification of chimerism samples. Specific for digital PCR is the sample-partitioning into a plethora of nano droplets or cavities. Each separate compartment contains nano liters of the sample of interest and during thermal cycling it functions as a separate PCR amplification chamber. Depending on how many copies of the target DNA molecules have been dispensed into the droplets or cavities (zero, one or more copies) a multitude of replicates is generated per single PCR run. Using Poisson statistics, the absolute number of starting copies can be determined very accurately. After thermal cycling each compartment is automatically analyzed and determined as positive or negative fraction. Because the digital PCR uses end-point detection of the amplification product, efficiency of the amplification is much less of a concern and calibration curves are not necessary.
Mentype® DigitalScreen represents a screening plate and allows the fast identification of biallelic short insertion/deletion polymorphisms (INDELs). The thereby identified markers can be used for subsequent DNA quantification with the kit Mentype® DigitalQuant.
Product References
- Fortschegger, M. et al. Detection and Monitoring of Lineage-Specific Chimerism by Digital Droplet PCR-Based Testing of Deletion/Insertion Polymorphisms. Biol Blood Marrow Transplant 26, 1218-1224, https://doi.org/10.1016/j.bbmt.2020.02.016 (2020)
- Stahl, T. et al. Digital PCR Panel for Sensitive Hematopoietic Chimerism Quantification after Allogeneic Stem Cell Transplantation. Int. J. Mol. Sci. 17. https://doi.org/10.3390/ijms17091515 (2016)
Resources
Ordering Information
Mentype® DigitalScreen
Size: 4 Plates (8 samples)
Cat. No.: 45-64610-0004
Status: RUO*
* RUO - Research Use Only products must be validated by the customer with clinically relevant material for diagnostic purposes.
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