Mentype® Nonaplex I PCR Amplification Kit
The cost-effective approach for DNA genotyping
Features
- Fast, robust and cost-efficient human identification
- Simultaneous amplification of 8 polymorphic STR markers and 1 sex-specific insertion-deletion locus
- Core STR-markers of German Forensic DNA Database, Interpol, and EDNAP
- Precise and reliable discrimination of alleles of low and mixed DNA material
Mentype® Nonaplex I was developed for the multiplex amplification of the eight core short tandem repeat (STR) markers of the German DNA database plus Amelogenin. The primer mix is optimized to provide a well-balanced intensity of all signals. The Mentype® Nonaplex I kit enables a fast and well-defined interpretation of STR profiles from blood samples, swabs, and forensic stains.
Biomarkers
Locus: Amelogenin X
Chromosomal Location: Xp22.1-22.3
Locus: Amelogenin Y
Chromosomal Location: Yp11.2
Locus: D3S1358
Chromosomal Location: 3p25.3
Locus: D8S1179
Chromosomal Location: 8q23.1-23.2
Locus: D18S51
Chromosomal Location: 18q21.3
Locus: D21S11
Chromosomal Location: 21q21.1
Locus: FGA (FIBRA)
Chromosomal Location: 4q28.2
Locus: SE33
Chromosomal Location: 6q14.2
Locus: TH01
Chromosomal Location: 11p15.5pter
Locus: vWA
Chromosomal Location: 12p13.31
Product Specifications
- Panel
- 8 STRs + Amelogenin
- Reactions
- 1 Multiplex PCR Reaktion pro Probe
- PCR controls
- 2 included (PC, NTC)
- Sample input
- 0.5 – 1.0 ng gDNA
- Detection limit
- 0.2 ng gDNA
- Turnaround time
- 4.5 h after nucleic acid preparation
- Detection
- Qualitative
- To be used with
- Standard thermal cycler + Thermo Fisher Genetic Analyzer
- Data analysis
- GeneMapper ID-X + specific templates
Scientific Background
The detection of STR markers in forensics is of crucial importance as these short tandem repeat sequences are highly variable in the human genome and thus exhibit high genetic diversity between individuals. This enables forensic scientists to create precise DNA profiles that allow the unambiguous identification of human genotypes.
Product References
- Chunli Wang et al. Generation of patient-derived IPSC lines from a girl with Combined Oxidative Phosphorylation Deficiency 23 (COXPD23) caused by compound heterozygous GTPBP3 variants. Stem Cell Research 61. https://doi.org/10.1016/j.scr.2022.102775 (2022)
- Janz, A. et al. CRISPR/Cas9-edited PKP2 knock-out (JMUi001-A-2) and DSG2 knock-out (JMUi001-A-3) iPSC lines as an isogenic human model system for arrhythmogenic cardiomyopathy (ACM). Stem Cell Research 53. https://doi.org/10.1016/j.scr.2021.102256 (2021)
- Janz, A. et al. Generation of two patient-derived iPSC lines from siblings (LIBUCi001-A and LIBUCi002-A) and a genetically modified iPSC line (JMUi001-A-1) to mimic dilated cardiomyopathy with ataxia (DCMA) caused by a homozygous DNAJC19 mutation. Stem Cell Research 46. https://doi.org/10.1016/j.scr.2020.101856 (2020)
- Deucher, A. et al. Rare Sequence Variation in the Genome Flanking a Short Tandem Repeat Locus Can Lead to a Question of “Nonmaternity”. JMD 12, 384-389. https://doi.org/10.2353/jmoldx.2010.090201 (2010)
- hHering, S. et al. Further sequence data of allelic variants at the STR locus ACTBP2 (SE33): Detection of a very short off ladder allele. International Congress Series K1288, 810-812. https://doi.org/10.1016/j.ics.2005.08.010 (2006)
- Hering, S. et al. Identification of more sequence variations in the D8S1179 locus. Forensic Science International 149, 275-278. https://doi.org/10.1016/j.forsciint.2004.07.011 (2005)
Resources
Ordering Information
Mentype® Nonaplex I
Size: 100 reactions
Cat. No.: 41-09113-0100
Status: RUO*
Mentype® Nonaplex I
Size: 400 reactions
Cat. No.: 41-09113-0400
Status: RUO*
*RUO - Research Use Only, not for diagnostic purposes.
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